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品牌: PicoQuant

應用: 光學量測

MicroTime 200 STED

Goods number:
In recent years, super-resolution microscopy has gained more and more attention. It has now evolved beyond the stage of development and permits to investigate biological systems that were formerly obscured by the diffraction limit of light. One of the most popular techniques for super-resolution imaging is Stimulated Emission Depletion (STED) microscopy. STED is usually performed with confocal microscopes and is therefore ideally suited to be added to the MicroTime 200. The integration of STED into the system has been driven towards highest robustness and ease-of-use. The system permits to perform STED microscopy without lengthy alignment preparations while still having the choice to modify the system and use the full capability of the open microscopy platform MicroTime 200.

產品敘述

Time-resolved Confocal Fluorescence Microscope with Super-resolution Capability

  • Complete confocal STED system based on inverted microscope body
  • Optical resolution below 50 nm
  • Excitation at 640 nm and optionally with additional lasers at 595 nm and 660 nm
  • Up to 6 truly parallel detection channels using SPADs or Hybrid-PMTs
  • Supports gated STED (gSTED) and gSTED-FCS
  • Piezo scanning for 2D- and 3D-(lifetime) imaging and accurate point positioning
  • Advanced easy-to-use data acquisition and analysis software SymPhoTime 64
  • Upgrade options for simultaneous AFM/FLIM/STED measurements
  • Supports all other methods available for the MicroTime 200, i.e., FLIM, FCS, FCCS, FLCS, FRET, etc.
  • NEW: FLIMbee galvo scanner add-on with outstanding flexibility in scanning speed and excellent spatial precision
  • NEW: scanning FCS available via line scan in x using FLIMbee galvo scanner

詳細規格

The MicroTime 200 STD is a time-resolved confocal microscope with single molecule sensitivity and super-resolution capabilities. Hence, numrous applications are possble with this instrument, including:

  • Stimulated Emission Depletion Microscopy (STED) / gated STED
  • Single Molecule Spectroscopy / Detection
  • Time-Resolved Fluorescence
  • Fluorescence Lifetime Imaging (FLIM)
  • Phosphorescence Lifetime Imaging (PLIM)
  • Fluorescence Correlation Spectroscopy (FCS)
  • Fluorescence Lifetime Correlation Spectroscopy (FLCS)
  • Foerster Resonance Energy Transfer (FRET)
  • Dual-focus Fluorescence Correlation Spectroscopy (2fFCS)
  • Scanning FCS (sFCS)
  • Pulsed Interleaved Excitation (PIE)
  • Fluorescence Anisotropy (Polarization)
  • Pattern Matching Analysis
  • Time-Resolved Photoluminescence (TRPL)
  • TRPL Imaging
  • Antibunching
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